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. 2020 Aug 8;6:73. doi: 10.1038/s41420-020-00308-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Coronal sections of the hippocampus were stained with anti-EAAC1 and anti-PV antibodies. Immunoreactivity was visualized using Alexa 488-conjugated secondary antibodies of anti-EAAC1 and Oyster 550 fluorescence-labeled primary antibodies of anti-PV. Scale bar, 50 µM; inset, enlarged areas. Scale bar, 5 µm. b Quantification analysis of PV immunoreactivity in (a). Fluorescence density of PV (red) and analysis by confocal microscopy. The data on PV staining quantification are expressed as the means ± S.E.M.; n = 10. **P < 0.01. c Quantification analysis of PV (red) clusters with EAAC1 (green) and EAAC1 clusters with PV in (a). The quantification data on EAAC1 and PV staining are expressed as the means ± S.E.M.; n = 16. d Quantification of the EAAC1 cluster number in (a). The results are presented as the means ± S.E.M.; n = 16. ***P < 0.001. e Quantification of the PV cluster number in (a). The results are presented as the means ± S.E.M.; n = 16. ***P < 0.001. f Immunoblot analysis of GAD65, GAD67, and PV in the hippocampus of CON and NMS rats. g Quantification analysis of GAD65, GAD67, and PV immunoreactivity in (f). The results are presented as the means ± S.E.M.; n = 8 (GAD 65, GAD67), n = 4 (PV).