Table 1.
Specificity of the primer/probe sets from in vitro SARS-CoV-2 infected cells. The Table shows the cycle threshold (Ct) of individual RT-qPCR reaction. The table shows representative data performed in duplicates from at least two independent experiments. “No Ct” indicates that no signal was detected over 40 cycles. nt: not tested.
| Primer/probe set | N1 | N3 | Ebo Std | RLP27 |
|---|---|---|---|---|
| RNA from non-infected cells | No Ct | No Ct | No Ct | nt |
| RNA from SARS-CoV-2 infected cells | 19 | 17.4 | No Ct | nt |
| Water samples collected upstream of the Montpellier WWTP | 36.4 | 37.6 | No Ct | 30.9 |
| No Ct | 37.3 | No Ct | 30.2 | |
| Water samples collected upstream of the Murviel-lès-Montpellier WWTP | No Ct | nt | nt | 39.0 |
| No Ct | nt | nt | 34.5 | |
| Sample collected in Montpellier household wastewater | No Ct | nt | nt | 24.6 |
| No Ct | nt | nt | 27.5 |