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. 2020 Apr 24;26(9):902–912. doi: 10.1111/cns.13383

Figure 2.

Figure 2

miR‐322 interacts with its target gene, NOX4. (A) Schematic representation of the NOX4 mRNA depicting miR‐322 binding sites in its 3’‐UTR. One predicted miR‐322 binding site (position 2437‐2460) is located in the 3’‐UTR of NOX4 mRNA. The effect of miR‐322 (B) and U6 (C) on the mRNA expression level at 48 hour after biotin‐miR‐322 transfection was detected using qRT‐PCR. (D) NOX4 mRNA level in the precipitates pulled down by biotin‐miR‐322 and biotin‐labeled negative control was detected using qRT‐PCR. (E) qRT‐PCR was used to detect the expression level of input NOX4 mRNA. All experiments were performed in triplicate. “**” and “****,” respectively, represented P < .01 and P < .0001, compared with the Bio‐con group (transfected with biotin‐labeled scrambled control miRNA)