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. 2020 Apr 24;26(9):902–912. doi: 10.1111/cns.13383

Figure 3.

Figure 3

miR‐322 suppresses NOX4 translation. (A) Levels of reporter activity as measured by analysis of the 3’UTR‐Luc, BS‐Luc, and Mut‐Luc luciferase reporters after overexpression of miR‐322. Left, plasmid schematic of different chimeric firefly luciferase‐NOX4 reporters. Luc, luciferase; BS, miR‐322‐binding site; Mut, mutation. Right, the luciferase activities levels of 3’UTR‐Luc, BS‐Luc, and Mut‐Luc reporters. C17.2 cells were transfected with different NOX4‐luciferase reporter plasmids, together with miR‐322 mimic or negative control. Levels of firefly and Renilla luciferase activities were assayed 48 hour later. Results were normalized to the Renilla luciferase activity. (B) miR‐322 levels in cells transfected with the miR‐322 mimic. Protein abundance (C) and mRNA levels (D) of NOX4 after miR‐322 mimic transfection. (E) miR‐322 levels in cells transfected with the miR‐322 inhibitor. Effect of the miR‐322 inhibitor on NOX4 protein (F) and mRNA (G) expression. The assay was repeated for three times. “*” and “**” represented P < .05 and P < .01, respectively