Figure 4.

miR‐322 attenuates NOX4‐triggered cell apoptosis. (A) The protein expression of cleaved caspase‐3, Bax, Bcl‐2, and NOX4 was determined by Western blot using β‐actin as an internal control. (B) Cleaved caspase‐3, Bax, Bcl‐2, and NOX4 protein levels were determined by Western blot after cells were transfected with NOX4 and/or the miR‐322 mimic. (C) Representative images of the TUNEL assay. Cells were transfected with the NOX4 full‐length expression plasmid, and cotransected with miR‐322 mimic or control mimic. Apoptotic cells were labeled by the TUNEL reagents and all cell nuclei were stained by DAPI. These assays were repeated three times. *P < .05, **P < .01, ***P < .001, ****P < .0001