FIGURE 4.

Astrocytes are reprogrammed into neural stem cell‐like cells after the transcription factors OCT4 and KLF4 were overexpressed in vitro. (A) Q‐PCR results of four transcription factors in astrocytes cultured for 3 d in Dox‐containing medium; (B) GFAP staining of astrocytes cultured in Dox‐containing medium for 0, 7, and 14 d, scale bar, 200 μm; (C‐F) protein expression levels of OCT4, KLF4 and GFAP were measured by Western blot analysis at day 14; (G) immunofluorescence stain of Nestin of astrocytes at day 14, scale bar, 200 μm. Data were expressed as mean ± SEM, unpaired Student's t test, **P < .01, ***P < .001, n = 5 per group