Table 1.
Differentiation potential of transposon-mediated induced pluripotent stem cells
| Species | Cell type | Transposon system | Reprogramming factors |
Differentiation |
Characterization of lineage specific differentiated cells through |
Chimera | Germline contribution | Ref. | ||
| In vitro | In vivo | Histology | Expression of gene/protein | |||||||
| Bat | Fetal fibroblasts | PB | Human OKSMNL + NR5A2, and bat-specific miR302/367 | EBs | Teratoma | Yes | Yes | NA | NA | Mo et al[27], 2014 |
| Buffalo | Fetal fibroblasts | PB | Human OSKMNL | EBs | NA | NA | Yes (ectoderm- NF-68 and Cytokeratin 8; mesoderm- MSX1 and endoderm- GATA4) | NA | NA | Kumar et al[32], 2019 |
| Cattle | Fetal fibroblasts | PB SB | Human OSKMNL Murine OSKM | EBs | Teratoma | Yes | Yes (Ectoderm- β III-Tubulin, Nestin; Mesoderm- Vimentin and Endoderm- AFP, GATA, PAX6 | NA | NA | Talluri et al[30], 2015 |
| Fetal fibroblasts | PB | Bovine OSKM | EBs | Teratoma | Yes | Yes (Ectoderm- β III-Tubulin; Mesoderm-α-SMA and endoderm- AFP) | NA | NA | Zhao et al[31], 2017 | |
| Horse | Fetal fibroblasts | PB | Murine OSKM | EBs | Teratoma | Yes | NA | NA | NA | Nagy et al[26], 2011 |
| Human | Skin fibroblasts | PB | OSKML | EBs, Keratinocyte | Teratoma | NA | Yes (ectoderm- K14; mesoderm- desmin and endoderm- AFP) | Ethically not allowed | Ethically not allowed | Igawa et al[116], 2014 |
| Fetal fibroblasts | SB | Murine OSKM | EBs | NA | NA | Yes (Ectoderm- β III-Tubulin, Nestin; Mesoderm- Vimentin and Endoderm- AFP | Ethically not allowed | Ethically not allowed | Davis et al[20], 2013 | |
| Monkey | Skin fibroblasts | PB | Monkey OSKMNL | EBs | Teratoma | Yes | Yes (Ectoderm- β III-Tubulin; Mesoderm- SMA and Endoderm- AFP | NA | NA | Debowski et al[28], 2015 |
| Mouse | Fetal fibroblasts | PB | Murine OSKM | EBs | Teratoma | Yes | NA | Yes | Yes | Yusa et al[115], 2009 |
| Fetal fibroblasts | SB | Murine OSKM | EBs | Teratoma | NA | Yes (cardiac-cTnT and desmin and neuronal- nestin and Tuj1) | Yes | Yes | Muenthaisong et al[21], 2012 | |
| Pig | Fetal fibroblasts | SB | Murine OSKM | Neuronal lineage | Teratoma | Yes | Yes | NA | NA | Kues et al[25], 2013 |
| Prairie vole | Fetal fibroblasts | PB | Murine OSKMNL | EBs | Teratoma | Yes | NA | NA | NA | Katayama et al[114], 2016 |
| Rat | Fetal fibroblasts | PB | OSKM | EBs, chondrocyte | NA | NA | NA | NA | NA | Ye et al[29], 2015 |
O: Oct4; S: Sox2; K: klf4; M: cMyc; N: Nanog; L: Lin28; EBs: Embryoid bodies; NA: Not applicable; PS: PiggyBac; SB: Sleeping beauty; AFP: α-Fetoprotein; SMA: Smooth muscle actin.