Figure 1.

Profiles of OxPCs in the AHs of EAU rats and uveitis patients. (A) Rat EAU was induced by an immunized bovine interphotoreceptor retinoid-binding protein (bIRBP) peptide with CFA to 7-week-old Lewis rats. Age-matched rats immunized without bIRBP were used as controls. EAU rats’ AHs were collected at 2 weeks after the immunization at the peak of intraocular inflammation. The AHs samples were analyzed by LC-MS/MS (4 rat eyes / sample). Error bars indicate standard deviation (n = 4). (B) the AHs collected from five senile patients with cataracts but without any ocular diseases and from five patients with cataracts with uveitis were classified as the control and uveitis groups, respectively. Both groups’ AHs were analyzed by LC-MS/MS as described in the Materials and Methods. Error bars indicate standard deviation (n = 5). Significance was determined using a nonpaired Student's t test. Asterisks indicate significant differences between the control and uveitis groups (P < 0.05). Formyl group-truncated OxPCs POBPC, POVPC, and PONPC denote 1-palmitoyl-2-(4’-oxo-butyroyl)-sn-glycero-3-PC, 1-palmitoyl-2-(5’-oxo-valeroyl)-sn-glycero-3-PC and 1-palmitoyl-2-(9’-oxo-nonanoyl)-sn-glycero-3-PC, respectively. Carboxyl group-truncated OxPCs PMPC, PGPC, and PAzPC denote 1-palmitoyl-2-malonyl-sn-glycero-3-PC, 1-palmitoyl-2-glutaryl-sn-glycero-3-PC, and 1-palmitoyl-2-azelaoyl-sn-glycero-3-PC, respectively.