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. 2020 Jun 11;61(6):23. doi: 10.1167/iovs.61.6.23

Figure 2.

Figure 2.

Effect of truncated oxidized phosphatidylcholines on ARPE-19 cells. (A) ARPE-19 cells were treated with 15 µM or 30 µM of four different truncated OxPCs, POVPC, PGPC, PONPC, and PAzPC, for 16 hours at 37°C as described in the Materials and Methods. After the treatment, a WST-1 assay was applied to determine the cytotoxicity through OxPC treatment. Error bars indicate standard deviation (n = 5). The viability of ARPE-19 cells treated without OxPC was defined as 100% (control). (B) The control medium indicates a culture supernatant of mouse AMs. Control medium + OxPC indicates that 30 µM OxPC was added when the control medium was applied to ARPE-19 cells. The pretreated OxPC medium indicates that OxPC was incubated on AMs for 4 hours, and the resultant supernatant was then applied to ARPE-19 cells. The viability of ARPE-19 cells treated with the control medium was defined as 100% (control). Error bars indicate standard deviation (n = 5). Significance was determined using a nonpaired Student's t-test. Asterisks indicate significant differences against the control medium (P < 0.01). A and B represent two independent experiments.