Figure 3.

Elimination of truncated oxidized phosphatidylcholine in the medium by mouse AMs. Four different kinds of 30 µM truncated OxPCs such as POVPC (POV), PGPC (PG), PONPC (PON), and PAzPC (Paz) were treated with mouse AMs for 1 and 4 hours at 37°C. The truncated OxPC found in the medium at each time point is shown by TLC (A) and quantified as described in the Materials and Methods. The truncated OxPC concentration in the medium at different time points was plotted against the incubation time (B). The OxPC concentrations in the cell and medium were determined from three different cultures. Error bars indicate standard deviation (n = 3). The values of Rf for POVPC, PGPC, PONPC, and PAzPC on the plate were 0.39, 0.24, 0.46, and 0.38, respectively. (C) Individual OxPCs were treated with the medium without mouse AMs for 4 hours at 37°C. The truncated OxPC found in the medium is shown by TLC (C). Little lyso-PC or fatty acid was detected in this study.