Figure 4.

Removal of PAzPC by mouse AMs obtained from wild-type and LPLA2 deficient mice. The cell culture, lipid, and protein analyses were carried out as described in the Materials and Methods. The cells (2.5 × 10⁵ per well) were treated in the presence or absence of 15 µM PAzPC. After the treatment for 4 hours, the media were collected and 100 µl was immediately used for lipid analysis. The PAzPC content in the medium is shown in the right panel. Error bars indicate standard deviation (n = 3). The cells were washed with PBS, fixed with methanol, and used for protein and lipid analysis. The PAzPC content in the cell is shown in the left panel. Error bars indicate standard deviation (n = 3). Significance was determined using a nonpaired Student's t-test. Asterisks indicate significant differences between the wild-type and LPLA2 deficient cells (*P < 0.05, **P < 0.01). Similar results were also obtained from another independent experiment. WT and KO denote wild-type and LPLA2 deficient mice, respectively.