Figure 8.

Wnt modulation significantly alters MYOC expression. Myocilin expression with Wnt modulation was documented by (A) qPCR (n = 3 donor strains), and (B) Western blotting (n = 4 donor strains). Both mRNA and proteins levels of MYOC were drastically reduced with Wnt activation. However, increases in MYOC expression after Wnt inhibition were statistically significant only at the protein level. The treatment regimen are as follows: − − = indicates no treatment; + − = indicates cells were treated with only LGK974; + + = indicates cells were first treated with LGK974 for 9 days following which they were treated with LY2090314 for 3 days; − + = corresponds to cells treated with only LY2090314. Asterisks (*) are used to show comparisons made my 1-way ANOVA followed by Dunnett's post hoc multiple comparison compared with control; *P < 0.05; **P < 0.01; ***P < 0.001. Hash symbol (#) denotes Student's t-test comparing results between “Wnt inhibition” and “Wnt activation after inhibition”; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001.