Figure 5.

Latency levels in infected mice. To analyze levels of latency in the TG of latently-infected mice, WT and IL17A^−/−, IL17RA^−/−, IL17RC^−/−, and IL17RA^−/−RC^−/− mice were infected in the eye as described in Figure 2 above. On day 28 PI, TG were harvested from the latently-infected mice. Quantitative RT-PCR was performed on individual TG from each mouse. GAPDH expression was used to normalize relative expression of LAT RNA in the TG. LAT copy number per TG were measured using pGEM5317, a LAT containing plasmid as we described previously.⁴⁹ Latency is based on 18 TG per group of mice. p-value was determined using a one-way ANOVA test.