Figure 5.
Gene expression patterns of inflammatory cytokines revealed by qPCR. The expression patterns of five inflammatory cytokine genes, including IL1B (A), IL6 (B), IL10 (C), IL23 (D), and TNF-α (E), were validated by qPCR. The experiment was performed in triplicate for each group and was repeated three times. The x-axis represents three groups: PBS-2, Af-2, and Af-5. The y-axis represents the relative expression of each gene in the different groups. The gene expression value in PBS-2 was set to 1.