Table 1.

Secondary metabolites and corresponding gene clusters of Pseudomonas strains.

Secondary metabolites	Gene involved in biosynthesis	Reference strain a	Locus number	JV222	JV395B	JV497	F113
Antimicrobial compounds
2,4‐diacetylphloroglucinol (DAPG)	phlIEDBCAFGH	P. kilonensis F113	PSF113_2457‐2464				X
Hydrogen cyanide (HCN)	hcnABC	P. kilonensis F113	PSF113_2367‐2369				X
		P. chlororaphis subsp. aureofaciens 30‐84	JV395B_v1_740005‐740007		X
			JV497_v1_90035‐90037			X
		Pseudomonas fluorescens Pf0‐1	JV222_v1_460055‐4600557	X
Pyrrolnitrin	prnDCBA	P. chlororaphis subsp. aureofaciens 30‐84	JV497_v1_130011‐130014			X
			JV395B_v1_1150076‐1150079		X
Phenazine	phzIRABCABFD + phzH	P. chlororaphis subsp. aurantiaca Pcho10	JV497_v1_250066‐250076			X
	phzIRABCABFD + phzO	P. chlororaphis subsp. aureofaciens 30‐84	JV395B_v1_1530032‐1530043		X
Alkylresorcinol	darR; darCBA	P. chlororaphis subsp. aureofaciens 30‐84	JV497_v1_210011‐210015			X
			JV395B_v1_120011;v1_1280003‐1280005		X
Putative Lankacidin	lkcABCDEFGJ; pqqFABCDEG	P. kilonensis F113	PSF113_3657‐3666; PSF113_5383‐5388				X
Quorum sensing related compounds
Homoserine lactone	phzI/phzR	P. chlororaphis subsp. aureofaciens 30‐84	JV497_v1_250066‐250067			X
			JV395B_v1_1530032‐1530033		X
	csaI/csaR	P. chlororaphis subsp. aureofaciens 30‐84	JV497_v1_100065‐100066			X
			JV395B_v1_760017‐760018		X
	aurI/aurR	P. chlororaphis subsp. aurantiaca StFRB508	JV395B_v1_1510126‐1510127		X
Auxin compounds
Auxin	iaaM	Pseudomonas moraviensis R28‐S	JV222_v1_690002	X
	iaaM	P. chlororaphis subsp. aureofaciens 30‐84	JV497_v1_320416			X
			JV395B_v1_1150001		X
	iaaM; amiE	P. kilonensis F113	PSF113_5381; PSF113_2053				X
Siderophore type compounds
Pyridine‐2,6‐thiocarboxylic acid (PDTC)	pdtCKPELMFGHIJON	P. kilonensis F113	PSF113_2605‐2618				X
Pyoverdine	pvdSL; pvdIJKDEONMP; fpvI; pvdA	P. kilonensis F113	PSF113_1749‐1750; PSF113_1836‐1847; PSF113_1856‐1860				X
	pvdSL; pvdLY; pvdAPMNOFEJID	P. chlororaphis subsp. aureofaciens 30‐84	JV395B_v1_1300021‐1300023; JV395B_v1_1250001‐1270001		X
			JV497_v1_201066‐201068; JV497_v1_200106‐210003			X
	pvdLS; pvdG‐pvdJ‐pvdO‐pvdP; pvdQfecR; fpvIpvdA	Pseudomonas moraviensis R28‐S	JV222_v1_510035‐510036; JV222_v1_260099‐260112; JV222_v1_330053‐330053; JV222_v1_270006‐270007	X
Achromobactin	cbrDCBA; acsABC; yhcA; acsEDF	P. chlororaphis subsp. aureofaciens 30‐84	JV395B_v1_940060‐JV395B_v1_940070		X
			JV497_v1_120382‐120392			X

Compounds	MIBiG	Locus number	JV222	JV395B	JV497	F113
Other antismash PKS and NRPS prediction (AntiSMASH)
NRP1	–	JV395B_v1_910035		X
		JV497_v1_120137			X
NRP2	–	JV395B_v1_800032‐800033		X
NRP3	–	JV497_v1_120298			X
NRP4	BGC0001135	JV222_v1_330154‐330156	X
NRP5	–	JV222_v1_260096‐260103	X
NRP6	BGC0000438	JV222_v1_310040‐310042	X
NRP7‐like	BGC0000387	JV222_v1_30009	X
		JV395B_v1_10008		X
		JV497_v1_10007			X
		PSF113_0201				X
PK1	BGC0000837	JV395B_v1_90058‐90061		X
		JV497_v1_30101‐30107			X
		JV222_v1_100073‐100076	X
		PSF113_0458‐0461				X
PK2	Butyrolactone	PSF113_3047‐3048				X

^aAnnotation of BGCs for F113 was performed according to Redondo‐Nieto et al. (2013). For P. chlororaphis strains JV395B and JV497, annotation was made by comparison (coverage > 80%; identity > 80%) with protein sequences of the reference strains P. chlororaphis subsp. aureofaciens 30‐84 (Loper et al., 2012), P. chlororaphis subsp. aurantiaca Pcho10 (Hu et al., 2014) or StFRB508 (Morohoshi et al., 2013). For P. koreensis JV222, Pseudomonas moraviensis R28‐S (Hunter et al., 2014) or Pseudomonas fluorescens Pf0‐1 (Silby et al., 2009) were used.