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. 2020 May 12;13(5):1489–1501. doi: 10.1111/1751-7915.13581

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© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 4 — MrNsdD expression during microsclerotium development and phenotypic characterization of microsclerotium development in liquid amended medium (AM) culture.

A. Transcription of MrNsdD during microsclerotium development. Samples were collected during the following stages: blastospores (36 h), hyphal period (60 h), microsclerotium initiation (72 h), microsclerotium formation (96 h) and microsclerotium maturation (120 h).

B. Development of microsclerotium in AM. Scale bar: 50 μm.

C. Phenotypic characterization of microsclerotium formation. Conidial suspension of the tested strains was inoculated in AM and cultured for 6 days.

D. Microsclerotium yields of the tested strains. Error bars represent standard error. *P < 0.05, **P < 0.01, when compared with WT or the results at 36 h.