Figure 6. Enrichment of ESFT-sEVs from clinical ESFT plasma samples.

(A) Total concentration of sEV’s isolated from 250 μL of pediatric ESFT (n = 10) and healthy plasma (n = 6). (B) Area Under the Curve (AUC) analysis (A) of total sEV counts in cases and controls following immuno-enrichment. (C) Detection of the EWS-ETS transcript by qRT-PCR, ^* denotes samples that crossed threshold and are considered positive, total of 7/10. cDNA from sEVs derived from TC-71 and Hs919.T cell lines served as positive and negative controls, respectively. (D) Localized and metastatic pediatric ESFT patient isolated sEVs demonstrating size threshold between 120–154 nm.