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. 2020 Aug 4;11(31):2959–2972. doi: 10.18632/oncotarget.27426

Figure 3. TQ reduces invasion and migration ability of 5FU-sensitive and 5FU-resistant HCT116 colorectal cancer cells.

Figure 3

HCT116 cells were seeded onto the Matrigel-coated membrane (invasion assay) (A) or the uncoated membrane (migration assay) (B) in the top chamber of the transwell and were either treated or not with 40 and 60 µM TQ respectively in the presence of FBS in the lower chamber. Cells that migrated/invaded to the lower chamber after 48hr were fixed with methanol, stained with H&E, counted and represented as number of migrating/invading cells compared to the control. Data represent an average of three independent experiments. The data are reported as mean ± SEM (* P < 0.05; ** P < 0.01; *** P < 0.001). (C) Representative confocal images and quantification of vimentin and E cadherin expression in 5FU-S and 5FU-R HCT116 colorectal cancer cells treated or not with 40 and 60 µM TQ, respectively. Data represent an average of three independent experiments and are reported as mean ± SEM (* P < 0.05; ** P < 0.01; *** P < 0.001). Scale bar for immunofluorescent images 20 µm.