Figure 2.

Effect of chidamide on the crizotinib-induced apoptosis and growth arrest of NSCLC cells. (A) Effect of chidamide (CHI), crizotinib (CRI), and chidamide-crizotinib cotreatment on the apoptosis of HCC827, H661, and Calu-3 cells. Cells were treated with chidamide (at 1/4 IC50) and crizotinib (0.5 or 1 µM) alone or in combination for 48 hr. DMSO was used as an agent control (Ctrl). Apoptotic cells were labeled with propidium iodide/Annexin V-FITC and analyzed by flow cytometry. (B) Effect of chidamide, crizotinib, and cotreatment on the colony formation of HCC827, H661, and Calu-3 cells. These cells were treated with the indicated concentrations of chidamide or crizotinib for 10-20 days. Colonies were stained with 0.1% crystal violet and photographed (left images). The results represent the mean of three independent experiments (right charts). **/***, p<0.01/0.001.