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. 2020 Jul 19;16(14):2595–2611. doi: 10.7150/ijbs.45886

Figure 5.

Figure 5

Effect of chidamide on the levels of c-MET expression in NSCLC cells. (A) Effects of chidamide treatment (0.25 µM for HCC827, 1 µM for H661, 0.2 µM for Calu-3; 4 hr) on the levels of c-MET expression in HCC827, H661, and Calu-3 cells in Western blotting (left images and middle charts) and qRT-PCR (right charts) analyses. (B) Effects of treatment with transcription, translation, and proteasome inhibitors (ActD, CHX, and MG132) on the chidamide-induced downregulation of c-MET expression in HCC827, H661, and Calu-3 cells by Western blotting. These cells were exposed to chidamide, ActD (5 µg/mL), CHX (50 µg/mL), or MG132 (10 µM) alone or cotreated with chidamide and ActD, CHX, or MG132 for 4 hr. (C) The level of c-MET mRNA in HCC827, H661, and Calu-3 cells cotreated with ActD. *, p<0.05; ns, not statistically significant.