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. 2020 Jul 19;16(14):2542–2558. doi: 10.7150/ijbs.45446

Figure 6.

Figure 6

Expression of TG2 in HCC cells is associated with IL-6/IL6R/STAT3 axis. (A) Recombinant IL-6 or HGF was added to the medium to treat Hep3B cells with a dose dependent manner. Indicated antibodies were used to test the protein level pSTAT3-S727, STAT3 and TG2. #, 5ng/mL; ##, 10ng/mL; *, IL-6 (5 ng/mL) + HGF (5 ng/mL); **, IL-6 (10 ng/mL) + HGF (10 ng/mL). (B) The corresponding neutralizing antibodies were added to medium after HCC cells were treated with recombinant IL-6 or HGF, independently. Indicated antibodies were used to test the protein level of TG2. (C) IL6R inhibitor, Tocilizumab (1 µM) was added to the medium and incubated for 12 hr. Indicated antibodies were used to test the protein levels of TG2, pSTAT3-S727, STAT3 and E-cad. (D) Small interference RNA target IL6R or STAT3 was used to deplete endogenous IL6R or STAT3 genes. Indicated antibodies were used to test the protein levels of TG2, IL6R, pSTAT3-S727, STAT3 and E-cadherin.

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