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. 2020 Jul 19;16(14):2542–2558. doi: 10.7150/ijbs.45446

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This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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STAT3 trans-activates TG2 gene expression to promote IL-6-induced EMT. (A) Human TG2 gene promoter (NC_000020.11) containing -1572/+217 was cloned and then truncations of four subclones: -1205/+217, -851/+217, -392/+217 and -35/+217 in pGL3B were used to construct the reporter plasmids of TG2 (TG2-Luc), and performed luciferase reporter assay. (B) A conserved STAT3 binding motif was located in the region of -317 to -307. Mutagenesis was performed to destroy STAT3 potential binding site. (C) Luciferase reporter assay was performed using wild type and mutant TG2 gene promoter. (D) Co-immuno-precipitation of pSTAT3-S727 and histone H1 was performed. (E) Chromatin-immuno-precipitation of pSTAT3-S727 targeting TG2 promoter was performed.

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