Table 1.
Primers used for qRT-PCR
| Gene | Forward Primer Sequences (5ʹ–3ʹ) | Reverse Primer Sequences (5ʹ–3ʹ) |
|---|---|---|
| CCL2 | CTCTCGCCTCCAGCATGAAA | TTTGCTTGTCCAGGTGGTCC |
| MMP9 | TCTATGGTCCTCGCCCTGAA | CATCGTCCACCGGACTCAAA |
| CCL5 | CAGTCGTCTTTGTCACCCGA | CGGGTGGGGTAGGATAGTGA |
| integrin-beta1 | CCGCGCGGAAAAGATGAAT | ATGTCATCTGGAGGGCAACC |
| E-cadherin | GCTGGACCGAGAGAGTTTCC | CAAAATCCAAGCCCGTGGTG |
| GAPDH | GAAGACGGGCGGAGAGAAAC | TGGAATTTGCCATGGGTGGA |
| IL-10 | TACGGCGCTGTCATCGATTT | TAGAGTCGCCACCCTGATGT |
| IL-4 | TCTTTGCTGCCTCCAAGAACA | GTTCCTGTCGAGCCGTTTCA |
| VEGFA | AGGGCAGAATCATCACGAAGT | AGGGTCTCGATTGGATGGCA |
| ALOX12 | CCATCTCACTGACCATTGTGG | CAGGCGGATGATGATGAGC |
| CCL22 | ATCGCCTACAGACTGCACTC | GACGGTAACGGACGTAATCAC |
| IL12p40 | AAAATAGATGCGTGCAAGAGAGG | GGGGAAGACCTGTGACTTGAG |
| CCR1 | ACTATGACACGACCACAGAGT | CCAACCAGGCCAATGACAAATA |
| CCR3 | TGGCATGTGTAAGCTCCTCTC | CCTGTCGATTGTCAGCAGGATTA |
| CCR4 | TCTCGCCAAGACACTGAACAG | GGCCCTGCATTCCTCAAGAAG |
| CCR5 | TTCTGGGCTCCCTACAACATT | TTGGTCCAACCTGTTAGAGCTA |
| IFN-Gamma | GGCCTCTACCACTATCTTCTCTC | ACACTGCTGAATTGACAAGGTTT |
| TGF-beta1 | GTAGCTCTGATGAGTGCAATGAC | CAGATATGGCAACTCCCAGTG |
| CSF1 | TGGCGAGCAGGAGTATCAC | AGGTCTCCATCTGACTGTCAAT |
| IL1beta | GA GCCCCAAAAGCAAGAGGAA | TGCGGGCATA CGGTTTCATC |