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. 2020 Aug 5;14:3165–3175. doi: 10.2147/DDDT.S250904

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© 2020 Liu et al.

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Juglanin prevented oxygen–glucose deprivation/reperfusion (OGD/R) in human bEnd.3 brain microvascular endothelial cells (HBMVECs). Cells were exposed to hypoxic conditions for 6 h, followed by exposure to reperfusion media for (24 h) in the presence or absence of juglanin (2.5, 5 μM). (A) Cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide (MTT) assay. (B) Release of lactate dehydrogenase (LDH) (^{#, *, &}P<0.01 vs previous column group).