Figure 1.

Experimental designs. A) EEG electrodes were implanted 10d before DFP administration. Two hours after DFP, diazepam was administered and a further 2h later DPO or vehicle was administered twice daily for three days. Video-EEG recorded for four weeks. B) Non-telemetered animals were used for behavioral data which was collected before and after the DFP exposure. DPO treatment was similar to the telemetry group. Animals were euthanized and brains processed for immunohistochemistry, Western blot, and multiplex assays. C, D) Initial SE severity quantification following acute DFP intoxication in telemetry animals and non-telemetry animals. In both groups, SE severity was scored in real-time and verified with video recordings. Data presented as mean ± SEM. There were no significant differences in the SE severity (convulsive or non-convulsive) between the vehicle and DPO treated groups in both telemetry and non-telemetry groups. (n=8 in telemetry group, n=12 in non-telemetry group, ns=non-significant).