Skip to main content
. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Mitochondrion. 2020 Feb 8;52:56–66. doi: 10.1016/j.mito.2020.02.001

Fig. 3.

Fig. 3.

Temozolomide has a dose-dependent decrease of mitochondrial respiratory function in cultured hippocampal neurons. The Seahorse X24 Flux Analyzer was used to measure neuronal Oxygen Consumption Rate (OCR) using the Cell Mito Stress Kit. Mitochondrial bioenergetic profile of cultured hippocampal neurons treated with (A) 250 μM TMZ and (E) 500 μM TMZ for 24 h and 48 h. Quantitative analysis of OCR levels reveals that 250 μM TMZ had a minimal effect on neuronal (B) basal OCR, (C) maximal respiratory rate, and (D) spare respiratory capacity. In contrast, 500 μM TMZ exerts a significant reduction of neuronal (F) basal OCR, (G) maximal respiratory rate, and (H) spare respiratory capacity. Maximum respiration was calculated by subtracting the non-mitochondrial respiration rate from the maximum rate measurement after FCCP injection. Spare respiratory capacity was calculated by subtracting the basal respiration rate from the maximum rate measurement after FCCP injection. Graphs represent mean ± SEM, n = 6 replicates per group. * p < 0.05, ** p < 0.01, *** p < 0.001.