Fig. 4.

In-vitro treatment with graded doses of Temozolomide reduces dendritic branching of cultured hippocampal neurons. (A) Representative images of hippocampal neurons treated with TMZ and immunolabeled for postsynaptic density-95 (PSD95). Neurons are superimposed over concentric Sholl circles (20 μm increments). (B,C) Quantification of dendritic branches at intersecting points with Sholl circles at increasing distance from the soma after (B) 4 h and (C) 24 h of treatment shows that TMZ reduces dendritic complexity at both timepoints. Data are presented as mean ± SEM, **** p < 0.0001, (n = 6 neurons per group). Scale bars, 20 μm. Red, PSD95.