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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Br J Haematol. 2020 Mar 2;190(3):405–417. doi: 10.1111/bjh.16530

Figure 5: SPHK2 inhibition downregulates Mcl-1 expression in LGL leukemia cells.

Figure 5:

A) Immunoblot analysis of pro-survival Bcl-2 family members in NKL cells after treatment with ABC294640. β-actin was used as a loading control. Bcl-2 family member expression was normalized to β-actin B) Immunoblot analysis of pro-survival Bcl-2 family members in TL-1 cells after treatment with ABC294640. β-actin was used as a loading control. Prosurvival Bcl-2 family member expression was quantified and normalized to β-actin C) Immunoblot analysis for Mcl-1 of PBMCs were isolated from representative NK-LGL (#1866) or T-LGL (#1947) patients and treated with ABC294640 for 12 hours. β2M was used as a loading control. D) Genetic knockdown of SPHK2, but not SPHK1, downregulates Mcl-1 protein expression in NKL and TL-1 cells. Scramble siRNA used as a control.