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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Mol Cancer Res. 2020 May 21;18(8):1244–1254. doi: 10.1158/1541-7786.MCR-20-0060

Figure 5. Prostate epithelial RON expression leads to increased 3D tumor cell growth in co-culture with macrophages.

Figure 5.

A) Percentage of BrdU positive TRAMP C2RE3 cells as determined by flow cytometry from cells cultured in COMPLETE or serum free media (SFM) in 2D cell culture conditions. B) Percentage of TRAMP C2RE3 cells cultured in complete or serum free media (SFM) in 2D cell culture conditions subjected to Annexin V/Propidium Iodide (PI) staining; Annexin V-/PI- (LIVE), Annexin V+/PI-, Annexin V+/PI+, and Annexin V-/PI+ (APOPTOTIC/DEAD). C) Percent of TRAMP C2RE3 spheres in each size classification. D) Fold change in the percent of spheres in each size classification in co-cultures of BMDM and Control or shRON TRAMP C2RE3 cells over the respective monocultures. *P < 0.05. E) Representative images of TRAMP C2RE3 spheres in monoculture or co-culture with BMDM (scale bar=500 μm).