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. Author manuscript; available in PMC: 2020 Dec 8.
Published in final edited form as: Nat Genet. 2020 Jun 8;52(8):819–827. doi: 10.1038/s41588-020-0639-9

Figure 4: Targeted demethylation in the absence of DNMT3 appears conserved in mice.

Figure 4:

a) Genome browser tracks for an orthologous locus in human ESCs and mouse EpiSCs show the conserved presence of DKO-DMRs following DNMT3 double knockout.

b) A comparison of DKO-DMRs identified in mouse DKO ESCs, DKO EpiSCs and a range of DKO-like Dnmt3a knockout mouse tissues. The number of DMRs derives from one experiment per condition. Homozygous Dnmt3a knockout mice (−/−) were generated by crossing Dnmt3a heterozygous mice (+/−). A representative photo of a WT and knockout mouse at day 23 is shown to highlight the expected runted phenotype in line with prior studies14. To the right, the number of DMRs identified for each cell type or each tissue is shown. Pluri. = Pluripotent.