Table 2.

Composition of immune microenvironment after RT in high-dose rate using flattening filter-free beams versus standard low-dose rate:

	T lymphocytes	T CD8 + lymphocytes	T CD4 + lymphocytes	NK lymphocytes	Treg lymphocytes
Control	3.26% [2.67; 6.81]	1.35% [1.02; 2.4]	1.37% [1.19; 2.87]	2.34% [2.13; 3.41]	0.38% [0.15; 0.77]
LDR (4 Gy/min)	19.2% [11.16; 26.71]	14.01% [8.85; 21.16]	3.84% [3.29; 4.86]	9.97% [8.93; 12.35]	2.21% [1.18; 2.67]
HDR (24 Gy/min)	17.56% [16.03; 25.33]	14.99% [8.83; 23.24]	3.94% [3.31; 6.48]	8.49% [6.97; 11.27]	1.79% [1.1; 2.11]
HDR VS LDR	p = 0.83	p = 0.84	p = 0.83	p = 0.36	p = 0.4
LDR VS Control	p = 0.011	p = 0.01	p = 0.02	p = 0.002	p = 0.003
HDR VS Control	p = 0.006	p = 0.01	p = 0.011	p = 0.03	p = 0.03

Percentages of various cellular types among total cells in CT26 tumor microenvironment, explored by flow cytometry at J7 after a single fraction of 16.5 Gy. All results represented in medians with interquartile range. Data compared using a Mann-Whitney non-parametric test. N = 5 samples for each condition. LDR: standard low-dose rate; HDR: High-dose rate.