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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: J Biol Inorg Chem. 2020 Jun 15;25(5):729–745. doi: 10.1007/s00775-020-01796-x

Figure 9. Flu-TSCZ depletes EPR-detectable Fe and increases EPR-detectable Mn.

Figure 9.

(a) Cu supplementation alone (red trace) did not impact the EPR signal at g = 4.3 relative to the untreated control cells (teal trace). (b) Treatment with 10 μM Flu-TSCZ with (red trace) or without (teal trace) Cu supplementation was not sufficient to impact the signal at g = 4.3. Increasing the concentration of Flu-TSCZ to 25 μM (c) or 100 μM (d) resulted in an attenuation of the g = 4.3 signal by approximately half and changed the signals in the g = 2 region, which are taken to be from Mn. Conditions: [Flu-TSCZ] is indicated in figure legends, [CuSO4] = 10 μM. Cells were treated for 6 h in YPD medium before analysis.