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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell. 2020 Jul 1;182(3):563–577.e20. doi: 10.1016/j.cell.2020.06.021

Figure 5. CD81 is required for de novo beige fat biogenesis.

Figure 5.

A) Illustration of the experiments in (B-E).

B) Ki67+ cells (%) in Lin−: Sca1+ stromal cells from the Ing WAT and Epi WAT in (A). n=5.

C) H&E staining and UCP1 immunofluorescent staining in the Ing WAT in (A). Arrowhead indicates lymph node (LN). Scale bar = 200 μm.

D) mRNA expression of indicated genes in the Ing WAT in (A). n=5.

E) OCR in the Ing WAT in (A). Control, n=3; CRISPRi-Cd81, n=4.

F) Rectal core body temperature of indicated mice following cold exposure. Control, n=6; CRISPRi-Cd81, n=5. *P < 0.05, **P < 0.01 by two-way repeated measures ANOVA with post hoc test by unpaired Student’s t-test.

G) Illustration of the experiments in (H-I).

H) H&E staining and UCP1 immunofluorescent staining in the middle region of Ing WAT in (G). Scale bar = 200 μm.

I) mRNA expression of indicated genes in the Ing WAT in (G). Control, n=5; PpargCD81 KO mice, n=4. (B, D, E, I) *P < 0.05, **P < 0.01 by unpaired Student’s t-test.

Data in (B, D, E, F, I) are represented as mean ± SEM.