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Pairwise Proximity Ligation Assay on normal adult human brain and Alzheimer’s Disease human brain. Fluorescent signals (red) indicate an interaction between LDLR and PGRMC1, LDLR and TMEM97, and TMEM97 and PGRMC1; indicating the trimeric complex is intact in normal and diseased human brains. Top 40X magnification, Bottom, expanded field of interest. Scale bars represent 25 μm.
