KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Cleaved Caspase 3 (Asp175), WB, dil:1/500; IF, dil: 1/50 | Cell Signaling Technology | Cat#9661; RRID: AB_2341188 |
| Cleaved Caspase 9 (Asp330) (Human Specific), WB, dil:1/1,000 | Cell Signaling Technology | Cat#9501; RRID: AB_331424 |
| BiP (C50B12), WB, dil:1/1,000 | Cell Signaling Technology | Cat#3177; RRID: AB_2119845 |
| CHOP (L63F7), WB, dil:1/1,000 | Cell Signaling Technology | Cat#2895; RRID: AB_2089254 |
| Rabbit Anti-PARP Monoclonal Antibody, WB, dil:1/1,000 | Cell Signaling Technology | clone 46D11, Cat#9532; RRID: AB_659884 |
| COX IV (3E11) Rabbit mAb antibody, WB, dil:1/30,000; IF, dil: 1/200 | Cell Signaling Technology | Cat#4850; RRID: AB_2085424 |
| Mouse Anti-β-Actin Monoclonal Antibody, WB, dil:1/50,000 | Sigma-Aldrich | clone AC-15, Cat#A1978; RRID: AB_476692 |
| CPT1A antibody [8F6AE9], WB, dil:1/10,000; IHC, dil: 1/200 | Abcam | Cat#ab128568; RRID: AB_11141632 |
| CPT1B antibody, WB, dil:1/1,000 | Abcam | Cat#ab104662; RRID: AB_10712608 |
| DGAT1 (H-255) antibody, WB, dil:1/1,000; IHC, 1/50 | Santa Cruz Biotechnology | Cat#sc-32861; RRID: AB_2090798 |
| PDIA1 (H-17) antibody, WB, dil:1/1,000 | Santa Cruz Biotechnology | Cat#sc-30932; RRID: AB_2156594 |
| Cytochrome c antibody, WB, dil:1/1,000 | BD Biosciences | clone 7H8.2C12, Cat#556433; RRID: AB_396417 |
| Anti-Perilipin 3/TIP47 antibody, IF, dil:1/200 | Abcam | Cat#ab47638 |
| Anti-mouse IgG, HRP-linked Antibody, WB, dil:1/1,000 – 5,000 | Cell Signaling Technology | Cat#7076; RRID: AB_330924 |
| Anti-rabbit IgG, HRP-linked Antibody, WB dil:1/1,000 – 5,000 | Cell Signaling Technology | Cat#7074; RRID: AB_2099233 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, IF dil: 1/500 | Thermo Fisher Scientific | Cat#A-11036, RRID: AB_143011 |
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, IF, dil: 1/500 | Thermo Fisher Scientific | Cat#A-11004; RRID: AB_2534072 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, IF, dil: 1/500 | Thermo Fisher Scientific | Cat#A-11034; RRID: AB_2576217 |
| Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, IHC dil:1/500 | Vector Laboratories | Cat#BA-2000, RRID: AB_2313581 |
| Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, IHC dil:1/500 | Vector Laboratories | Cat#BA-1100, RRID: AB_2336201 |
| Biological Samples | ||
| Human GBM TMA | Dr. Craig Horbinski, Northwestern University (previously at University of Kentucky) | https://www.feinberg.northwestern.edu/faculty-profiles/az/profile.html?xid=33419 |
| Human GBM patient samples | Department of Pathology at the OSU Medical Center | https://pathology.osu.edu/ |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Paraformaldehyde | Sigma-Aldrich | Cat#P6148 |
| Glutaraldehyde solution | Sigma-Aldrich | Cat#G5882 |
| Puromycin dihydrochloride | Sigma-Aldrich | Cat#P8833 |
| EGF, Epidermal Growth Factor human | Sigma-Aldrich | Cat#E9644 |
| Heparin sodium salt | Sigma-Aldrich | Cat#H3393 |
| Triton X-100 | Sigma-Aldrich | Cat#T8787 |
| Poly-L-lysine hydrobromide | Sigma-Aldrich | Cat#P5899 |
| Laminin | Sigma-Aldrich | Cat#L2020 |
| Stearoyl-L-carnitine (C18-Carnitine) | Sigma-Aldrich | Cat#61229 |
| Oligomycin A | Sigma-Aldrich | Cat#75351 |
| Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) | Sigma-Aldrich | Cat#C2920 |
| Rotenone | Sigma-Aldrich | Cat#R8875 |
| N-Acetyl-L-cysteine | Sigma-Aldrich | Cat#A7250 |
| Pepstatin A | Sigma-Aldrich | Cat#P5318 |
| Leupeptin | Sigma-Aldrich | Cat#L2884 |
| Phenylmethanesulfonyl fluoride (PMSF) | Sigma-Aldrich | Cat#P7626 |
| DL-Dithiothreitol (DTT) | Sigma-Aldrich | Cat#43819 |
| Calpain Inhibitor I (ALLN) | Sigma-Aldrich | Cat#A6185 |
| Polybrene | Sigma-Aldrich | Cat#H9268 |
| Butylated hydroxytoluene | Sigma-Aldrich | Cat#W218405 |
| TLC Silica gel | Sigma-Aldrich | Cat#1168350001 |
| B-27™ Supplement (50X), minus vitamin A | Thermo Fisher Scientific | Cat#12587010 |
| TrypLE™ Express Enzyme (1X), no phenol red | Thermo Fisher Scientific | Cat#12604021 |
| MitoTracker™ Red FM | Thermo Fisher Scientific | Cat#M22425 |
| Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix | Thermo Fisher Scientific | Cat#A1413301 |
| MitoTracker™ Green FM | Thermo Fisher Scientific | Cat#M7514 |
| CellROX™ Deep Red | Thermo Fisher Scientific | Cat#C10422 |
| TRIzol™ Reagent | Thermo Fisher Scientific | Cat#15596018 |
| Rhodamine 123 | Thermo Fisher Scientific | Cat#R302 |
| BODIPY 493/503 | Thermo Fisher Scientific | Cat#D3922 |
| RIPA buffer | Fisher Scientific | Cat#NC9484499 |
| Recombinant Human FGF | R&D Systems | Cat#4114-TC-01M |
| Etomoxir | R&D Systems | Cat#4539 |
| DGAT1 inhibitor A9–22500 | MedChemExpress LLC | Cat#HY-10038 |
| DGAT2 inhibitor PF-06424439 | Sigma-Aldrich | Cat#PZ0233 |
| C16:0 carnintine | Avanti Polar Lipids | Cat#870851P-5mg |
| Stearoyl-L-carnitine | Sigma-Aldrich | Cat#61229 |
| C18:1(Δ9-cis) Carnitine | Avanti Polar Lipids | Cat#870852P-5mg |
| Palmitic acid | Sigma-Aldrich | Cat#P0500 |
| Stearic acid | Sigma-Aldrich | Cat#S4751 |
| Oleic acid | Sigma-Aldrich | Cat#O1383 |
| cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail | Roche | Cat#11836170001 |
| phosphatase inhibitor tablets | Roche | Cat#4906845001 |
| Nitrocellulose Membrane | Bio-rad | Cat#162–0112 |
| Amersham™ ECL™ Western Blotting Detection Reagents | GE Healthcare | Cat#RPN2106 |
| VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) | Vector Laboratories | Cat#PK-4000 |
| Vector® NovaRED® Substrate Kit, Peroxidase (HRP) | Vector Laboratories | Cat#SK-4800 |
| Hematoxylin | Vector Laboratories | Cat#H-3401 |
| iQ™ SYBR® Green Supermix | Bio-rad | Cat#170–8882 |
| polyethylenimine | Polysciences | Cat#23966 |
| Lenti-X Concentrator | Clontech | Cat#631232 |
| Tet System Approved FBS | Clontech | Cat#631106 |
| Doxorubicin hydrochloride | Sigma-Aldrich | Cat# D1515 |
| 4μ8C | Selleckchem | Cat#S7272 |
| GSK2656157 | Selleckchem | Cat#S7033 |
| Charcoal-stripped FBS premium | Thermo Fisher Scientific | MT35072CV |
| Luciferin | Perkin Elmer | Cat#122796 |
| Critical Commercial Assays | ||
| qPCR Lentivirus Titration(Titer) Kit | Applied Biological Materials | Cat#LV900 |
| Qproteome Mitochondria Isolation Kit | Qiagen | Cat#37612 |
| iScript™ cDNA Synthesis Kit | Bio-rad | Cat#170–8891 |
| Experimental Models: Cell Lines | ||
| Human GBM cell: U87 | ATCC | Cat#ATCC® HTB-14 |
| Human GBM cell: T98 | ATCC | Cat#ATCC® CRL-1690 |
| Human GBM cell: U-251 | Sigma-Aldrich | Cat#09063001 |
| Human hepatocellular carcinoma cell: HepG2 | ATCC | Cat#ATCC® HB-8065 |
| Human GBM cell: U87/EGFRvIII | A kind gift from Dr. Paul Mischel (UCSD) | N/A |
| Human GBM cell: U87/EGFRvIII-luc | Generated in Dr. Balveen Kaur’s lab at OSU (currently working at UTHealth) | N/A |
| Human GBM primary cell: GBM30 | Originally generated in Dr. Ichiro Nakano’s lab at OSU (currently working at UAB) | N/A |
| Human GBM primary cell: GBM30-luc | Generated in Dr. Balveen Kaur’s lab at OSU (currently working at UTHealth) | N/A |
| Human GBM primary cell: GBM83 | Originally generated in Dr. Ichiro Nakano’s lab at OSU (currently working at UAB) | N/A |
| Human GBM primary cell: GBM169 | Originally generated in Dr. Ichiro Nakano’s lab at OSU (currently working at UAB) | N/A |
| Experimental Models: Organisms/Strains | ||
| Athymic nude (NCr-nu/nu) mice | OSU Target Validation Shared Resource | https://u.osu.edu/ccclabs/shared-resources-and-cores/ |
| Oligonucleotides | ||
| Primers for DGAT1, DGAT2 and 36B4, see Table S1 | This paper | N/A |
| Oligonucleotides for constructing inducible shRNA plasmids, see Table S2 | This Paper | N/A |
| Recombinant DNA | ||
| pLKO-Tet-On vector | Wiederschain et al.,2009 | Addgene, Cat#21915 |
| control shRNA | Sigma-Aldrich | Cat#SHC002 |
| shDGAT1–1 | Sigma-Aldrich | Cat# TRCN0000236207 |
| shDGAT1–2 | Sigma-Aldrich | Cat# TRCN0000036151 |
| shDGAT2–1 | Sigma-Aldrich | Cat# TRCN0000005193 |
| shDGAT2–2 | Sigma-Aldrich | Cat# TRCN0000424717 |
| psPAX2 | Addgene | Cat#12260 |
| pMD2.G | Addgene | Cat#12259 |
| lentiCRISPR-v2 | Addgene | Cat#52961 |
| Software and Algorithms | ||
| ZEN 2 (blue edition) | Zeiss | https://www.zeiss.com/microscopy/us/downloads.html |
| AxioVision Rel. 4.8.2 | Zeiss | https://www.zeiss.com/microscopy/us/downloads.html |
| LSM version 4.2 | Zeiss | https://www.zeiss.com/microscopy/us/downloads.html |
| Sequence Detection System v2.4 | Applied Biosystems | https://www.thermofisher.com/order/catalog/product/4350490#/4350490 |
| Seahorse Wave Controller 2.6 | Agilent | https://www.agilent.com/ |
| SPOT 5.2 | SPOT IMAGING | http://www.spotimaging.com/software/ |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Fiji, ImageJ | ImageJ | https://imagej.net/Downloads |
| ImageScope v11.1.2.760 | Aperio Technologies | https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/ |
| GlioVis | Robert L. Bowman and et al., 2017 | https://gliovis.shinyapps.io/GlioVis/ |
| SAS 9.4 | SAS | https://www.sas.com/en_us/software/sas9.html |
| Other | ||
| L-Glutamine | Thermo Fisher Scientific | Cat#25030081 |
| Sodium Pyruvate | Thermo Fisher Scientific | Cat#11360070 |
| Dulbecco’s modified Eagle’s medium | Corning | Cat#15–013-CV |
| DMEM (Dulbecco’s Modified Eagle’s Medium)/Hams F-12 50/50 Mix | Corning | Cat#90–090-PB |
| DMEM without Glucose, Glutamine, Sodium Pyruvate | Corning | Cat#17–207-CV |
| D-(+)-Glucose solution | Sigma-Aldrich | Cat#G8644–100ML |
| Doxycycline diets | ENVIGO | Cat#TD.01306 |