Table 1.

Shows The cumulated micronucleus frequencies of each concentration of the test compounds as well as the negative control after 24 h of treatment and 24 h of recovery (3 h of treatment and 45 h of recovery for clastogens that require metabolic activation), the total number of cells assessed and the statistical results including the p-values and functions

Compound	Concentration	Number of cells	Mean micronucleus frequency	p-value
4-Nitroquinoline R2 = 0.9811 y = 0.7151 + 18.6781x	Control	39,098	0.679
	0.025 µM	7647	1.364	Rp = 0.003
	0.05 µM	24,734	1.708	Rp < 0.001
	0.1 µM	23,654	2.247	R p < 0.001
	0.2 µM	17,439	7.500	R p < 0.001
	0.3 µM*	2654	9.910	Rp = 0.002
Ethyl methanesulfonate R2 = 0.9313 y = 0.4378–0.1265x + 0.0485x^2	Control	12,905	0.415
	1.28 mM	13,367	0.439	Tp = 0.409
	2.56 mM	15,157	0.320	Tp = 0.171
	3.84 mM	11,395	0.733	Rp = 0.016
	5.12 mM	8533	1.048	Rp < 0.001
Colchicine R2 = 0.9878 y = 0.7468 + 0.0028x + 0.0012x^2–1.3917E-005x^3	Control	29,834	0.778
	0.031 µM	25,320	0.847	Rp = 0.004
	0.062 µM	23,577	1.464	Rp < 0.001
	0.125 µM	12,936	2.200	Rp < 0.001
	0.188 µM*	11,894	2.032	Rp < 0.001
Diethylstilbestrol R2 = 0.9923 y = 0.5098–0.0087x + 0.0003x^2 + 2.1490E-007x^3	Control	18,969	0.483
	15 µM	7399	0.416	Tp = 0.887
	30 µM	15,238	0.746	Rp = 0.002
	45 µM	6310	0.636	Tp = 0.051
	60 µM	11,340	1.011	Rp < 0.001
	90 µM*	5796	2.469	Rp < 0.001
	120 µM*	2175	4.202	Rp < 0.001
Aflatoxin B1 + ewoS9R R2 = 0.9832 y = 0.5807 + 5.0574x− 2.017x^2	Control	3449	0.377
	0.00625 mM	4030	0.596	Tp = 0.026
	0.0125 mM	4187	0.549	Tp = 0.116
	0.025 mM	3244	0.956	Tp = 0.001
	0.05 mM	2840	0.915	Tp = 0.012
	0.1 mM	3491	0.802	Tp = 0.002
	0.2 mM	2307	1.517	Tp < 0.001
	0.4 mM	2062	2.279	Rp = 0.004
	0.8 mM	1293	3.326	Rp = 0.004
	1.6 mM	793	3.531	Rp = 0.010
Aflatoxin B1 + S9 mix R2 = 0.984 y = 0.7125 + 5.0221x-1.9428x^2	Control	5550	0.631
	0.00625 mM	4290	0.583	Tp = 0.503
	0.0125 mM	3495	1.059	Tp = 0.085
	0.025 mM	3346	0.837	Rp = 0.126
	0.05 mM	4395	0.865	Tp = 0.465
	0.1 mM	2673	1.085	Rp = 0.067
	0.2 mM	3263	1.502	Tp = 0.004
	0.4 mM	2237	2.414	Rp = 0.002
	0.8 mM	2281	3.595	Rp = 0.004
	1.6 mM	2367	3.718	Rp = 0.002
Cyclophosphamide + ewoS9R R2 = 0.8747 y = 0.5736 + 0.0067x− 1.1467E−005	Control	4046	0.420
	1.132 µM	3702	0.594	Tp = 0.091
	2.266 µM	3644	0.302	Rp = 0.180
	4.531 µM	2570	0.817	Rp = 0.015
	9.063 µM	3307	0.605	Rp = 0.065
	18.125 µM	2340	0.812	Rp = 0.126
	36.25 µM	2871	1.219	Rp = 0.002
	72.5 µM	2156	1.855	Rp = 0.004
	145 µM	3477	1.179	Rp = 0.002
	290 µM	3008	1.496	Rp = 0.004
Cyclophosphamide + S9 mix R2 = 0.9922 y = 0.8913 + 0.0188x− 3.2630E-005	Control	2747	0.837
	1.132 µM	3080	0.812	Tp = 0.711
	2.266 µM	2494	1.002	Tp = 0.590
	4.531 µM	2333	0.986	Rp = 0.937
	9.063 µM	2486	1.046	Tp = 0.667
	18.125 µM	2560	1.289	Tp = 0.018
	36.25 µM	2169	1.245	Tp = 0.073
	72.5 µM	2144	1.726	Tp = 0.017
	145 µM	2397	2.545	Rp = 0.002
	290 µM	2111	3.316	Rp = 0.002
DMSO R2 = 0.9907 y = 0.1637x + 0.4119	Control	15,961	0.407
	0.25%	12,772	0.469	Rp = 0.912
	0.5%	13,704	0.492	T p = 0.159
	1.0%	10,067	0.559	Tp = 0.033
	2.0%*	8981	0.746	Rp = 0.013
Cannabidiol R2 = 0.8063 y = 0.0681x + 0.3034	Control	10,080	0.387
	0.159 µM	11,512	0.487	Tp = 0186
	0.318 µM	10,423	0.441	Rp = 0.624
	1.590 µM	9392	0.532	Tp = 0.06
	3.180 µM	8857	0.700	Rp = 0.126

*Cytotoxicity threshold concentrations not tested repeatedly