Table 1.
siRNA Metabolite Profile Change in Rat Liver Homogenate during a 48-h Incubation
| Time Point (h) | Sense Strand Metabolites (%) |
Antisense Strand Metabolites (%) |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intact | −1 GalNAc | −2 GalNAc | −3 GalNAc | -PEG | -Branch | 3′n-1 -Branch | Intact | 3′ n-1 | 3′ n-2 | 3′ n-3 | 3′ n-4 | 3′ n-5 | 3′ n-6 | 5′ n-1 | |
| 1 | 98.0 | 1.6 | 0.3 | ND | ND | 0.1 | ND | 95.9 | 1.2 | 0.2 | 0.4 | 0.2 | ND | ND | 2.1 |
| 6 | 78.2 | 16.1 | 1.9 | ND | 0.1 | 0.1 | 3.6 | 76.8 | 11.7 | 0.4 | 0.6 | 4.9 | 1.9 | 1.6 | 2.1 |
| 24 | 31.8 | 32.8 | 20.4 | 10.5 | 0.1 | ND | 4.4 | 53.6 | 16.8 | 0.6 | 3.5 | 10.1 | 13.1 | 0.9 | 1.4 |
| 48 | 16.1 | 22.2 | 25.3 | 32.4 | 0.1 | ND | 3.9 | 49.5 | 17.4 | 0.6 | 2.3 | 12.8 | 14.6 | 1.6 | 1.2 |