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. 2020 Jul 25;10(20):9425–9442. doi: 10.7150/thno.43315

Figure 1.

Figure 1

Characterization of human adipose mesenchymal stem cells (AMSC)- and GDNF-transfected AMSCs (GDNF-AMSC)-derived exosomes. (A) AMSCs were plastic-adherent, with fibroblastic morphology. Fluorescence expression of lentiviral vector in GDNF-transfected AMSCs at 72 hours. Approximately 70% of AMSCs expressed the GFP gene 72 hours after transfection, as indicated by the green fluorescence. Scale bar: 100 µm. (B) Quantitative real-time PCR (qRT-PCR) verification of upregulated GDNF mRNA expression in AMSCs after GDNF transfer. The results are shown as fold changes. ***P < 0.001 compared with controls. (C) Flow cytometric analysis of surface markers on AMSCs. (D) Representative images of AMSCs induced to differentiate into adipogenic and osteogenic lineages: (upper) oil red O staining for adipocytes; (lower) alizarin red S staining for osteocytes. (E) Representative Western blot images of CD9, CD63 and CD81 protein expression in the kidney cortex. β-Actin was used as the loading control. (F) Appearance of exosomes by transmission electron microscopy. Scale bar: 50 nm. (G) The size of the exosomes secreted from GFP-AMSCs and GDNF-AMSCs was measured by NanoSight analysis.