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. 2020 Jul 11;10(20):9066–9082. doi: 10.7150/thno.45349

Figure 5.

Figure 5

AJUBA promotes EMT in HCC. The relative mRNA (A, B) and protein (C) expression levels of EMT markers (E-cadherin, β-catenin, N-cadherin, and vimentin) in AJUBA-overexpressed and AJUBA-silenced cells. The results shown in (A) and (B) are representative of three independent experiments. All error bars show standard error of the mean. *P < 0.05 and **P < 0.01. (D) The expression levels of Akt, p-Akt, p-GSK-3β, Snail, and GAPDH (loading control) measured by western blotting. (E-F) IF staining of β-catenin and vimentin in the indicated cell lines. Nuclei were counterstained with DAPI (magnification: 600×).