Figure 2.

NAC Prevented DEHP-induced IR. A-B. The representative western blot images and quantification of oxidative stress-related genes (n = 3 mice per group). The total protein was normalized by Gapdh and the protein expression of Nrf2 in nuclear were normalized by Lamin B1. C. The calculated GSH/GSSG ratio in serum (n = 5 mice per group). The serum levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were shown in Figure S2C-D. D. The serum level of H₂O₂ (n = 5 mice per group). E. The calculated GSH/GSSG ratio in SkM (n = 5 mice per group). The levels of GSH and GSSG in SkM were shown in Figure S2E-F. F. The content of H₂O₂ normalized to protein content in SkM (n = 5 mice per group). G. The fasting blood glucose (n = 10 mice per group). H. The IPGTT (n = 5 mice per group). The AUC of the IPGTT were shown in Figure S2G. I. ITT (n = 5 mice per group). J. The KITT obtained in ITT (0-30min). K-L. The representative western blot images (K) and quantification (L) of insulin-stimulated pAkt and the Glut4 translocation in SkM (n = 3 mice per group). The basal levels (without insulin stimulation) of pAkt and mGlut4 was shown in Figure S2H. M-N. The expression and phosphorylation of Insr and Irs1 in SkM. Quantitative results were normalized by Gapdh (n = 3 mice per group). O. The expression of miR-200 family in SkM of mice (n = 4 mice per group). U6 was used to normalized miRNA expression. P. The putative sequence interactions between miR-200a (miR-141) and 3'UTR of Insr and Irs1, respectively. Mmu, mouse; Hsa, human. All data were presented as the mean ± SEM. *P < 0.05 control mice vs. DEHP-exposed mice, **P < 0.01 control mice vs. DEHP-exposed mice. #P < 0.05 DEHP-exposed mice vs. DEHP-exposed mice co-treated with NAC, ##P < 0.01 DEHP-exposed mice vs. DEHP-exposed mice co-treated with NAC.