Figure 3.

Bioluminescence imaging of peripheral immune cells in a mouse neuroinflammation model using intracranial LPS injection. (A) Flow cytometry analysis of immune cells from splenocytes of C57BL/6 (wild type) and C57BL/6.Luc^Tg (reporter transgenic) mice. (B) Semiquantitative analysis of luciferase-expressing mouse splenocytes from C57BL/6.Luc^Tg. The intensity of luciferase signal and the number of cells were highly correlated (R²=0.9991) (C) Schematic representation of experimental design using luciferase-expressing splenocytes to monitor peripheral immune cell infiltration in the brain of a mouse with neuroinflammation induced by intracranial LPS injection. (D) Representative biodistribution of luciferase-expressing splenocytes in mice 4 days after intracranial injection of saline or LPS (N=2 for each treatment).