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. 2020 Jul 14;10(20):9186–9199. doi: 10.7150/thno.46825

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GYS1 regulates p65 via RPS27A. (A) Co-immunoprecipitation assays detected no interaction between GYS1 and p65. Cell lysate was immunoblotted with anti-GYS1 and anti-p65. (B) STRING database revealed the protein mediating the interaction between GYS1 and p65 (RELA). (C) Venn diagram indicating that RPS27A was identified by both IP-mass spectrum and the STRING database. (D) The silencing effect of RPS27A siRNAs was validated by qRT-PCR. (E) Western blot revealed the expression of p-p65 in the cytoplasm and nucleus. β-Actin and Lamin B were used as internal references in the cytoplasm and nucleus, respectively. (F) IF images indicating the subcellular localization of p65 (red). The nuclear outline was stained by DAPI (blue) in Caki-2 cells. (G) Expression of p65 and p-p65 was measured by western blotting following GYS1 overexpression or silencing in RPS27A-depleted cells. Statistical data are represented as the mean ± SD. *P < 0.05.