Figure 7.

GYS1 is synergistic with sunitinib in ccRCC cells. (A) Cell viability was detected by CCK8 assay at 48 h after sunitinib treatment. IC₅₀ was calculated using GraphPad Prism software (GraphPad, Inc., La Jolla, CA, USA). (B) Flow cytometry showed apoptotic cells by Annexin V/PI double staining in Caki-2 cells. (C) Western blotting revealed the protein expression of the GYS1 and NF-κB pathways 24 h after sunitinib treatment. (D) Protein level of p65 and p-p65 was measured after treatment with GYS1 siRNAs and sunitinib. (E) Schematic diagram showing the molecular regulation mechanisms in our study. Statistical data are represented as the mean ± SD. *P < 0.05.