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. 2020 Jul 11;10(20):9083–9099. doi: 10.7150/thno.46467

Figure 6.

Figure 6

Selene was specifically enriched in the mitochondria through TLR4/TRAF3/MFN1 protein complex-mediated membrane fusion. A. Results of potential bridge-linked protein analysis from FpClass database. B. Pull-down assay results (IP by the anti-Flag beads) of the input, control vector, and Flag-TLR4 overexpression groups detected via silver staining to identify proteins interacting with TLR4. C-E. Duolink PLA results of TLR4/TRAF3 and TRAF3/MFN1 after Selene treatment (Selene-L 10 µM, Selene-H 15 µM). F. Whole-cell lysates from OVCAR-3 were immunoprecipitated by anti-TRAF3, anti-TLR4, anti-MFN1, and anti-OPA1 antibodies, followed by Western blot with antibodies against the indicated proteins to verify the interactions of proteins. G. Cell lysate was separated by FPLC. Then, TRAF3, TLR4, and MFN1 in the fractions were detected using Western blot. Results are shown as means ± SD (*P < 0.05, **P < 0.01).