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. 2020 Apr 30;6(2):265. doi: 10.18063/ijb.v6i2.265

Figure 4.

Figure 4

Cell growth and mineralized matrix deposition on polymerized high internal phase emulsions (polyHIPE) scaffolds. (A) Metabolic activity over 14 days, 100 wt% significantly higher than plain on day 0 and 14 (P < 0.05). (B) Calcium deposition on days 7 and 14. Typical mineral staining for each condition shown immediately below each bar. Plain significantly higher than 50 wt% and 100 wt% on day 7 (P < 0.05), no significant differences by day 14. (C) Collagen deposition on days 7 and 14. Typical collagen staining for each condition showed immediately below each bar. 50 wt% had significantly less collagen than 100 wt% on day 7 (P < 0.05), no significant differences by day 14. Matrix staining is confined to the outer surfaces on plain polyHIPEs at both time points, whereas in alginate-leached polyHIPEs, penetration into the bulk of the material is visible.