Figure 9.

Histological sections of defect regions treated without bone morphogenetic protein 2 after 6 weeks. (A) Hematoxylin and eosin (H&E) stained sections of diamond scaffold, (B) H&E stained sections of cubic scaffold with the inset figures showing fibrous tissue in the pores and arrows indicating osteoblast cells lining the edges of the diamond glass scaffold strut, (C and D) magnified images of different regions of diamond scaffold showing fibrous connective tissue, newly formed bone tissue, and remaining glass, (E) Masson’s trichrome stain showing pocket of mineralized bone tissue in the pore and the new bone tissue (red) surrounding the glass filament indicated by dotted arrow, (F) Trichrome stain showing mineralized bone tissue formed adjacent to host bone tissue and from the bottom side of the defect (above dura matter). N – new mineralized bone, O – original host bone, G – remaining glass, F – fibrous connective tissue.