Supplementary Figure 10.

(A-B) SEM images of L929 cells on the surfaces of HAc-Alg and HAc-Alg/30wt% CaP after 3 days of culturing and (C) CLSM images of L929 fibroblasts cultured on HAc-Alg and HAc-Alg/30wt% CaP after 3 days of culturing. Cells didn’t fully cover the surface of HAc-Alg/30wt% CaP after 3 days of culturing, showing significant local variation of cell density. On the other hand, cells on HAc-Alg didn’t attach to the surface well, forming cell clusters. Cell morphologies of two hydrogel surfaces are also different: globular cells on HAc-Alg vs. elongated and flattened cells on HAc-Alg/30wt% CaP.