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. 2020 Apr 2;6(2):258. doi: 10.18063/ijb.v6i2.258.

Figure 6.

Figure 6

Cytocompatibility of hyaluronic acid-alginate (HAc-Alg) and HAc-Alg/30 wt% calcium phosphate (CaP) hydrogels using L929 fibroblasts. (A) Scanning electron microscope images of cells attached to the surfaces of HAc-Alg and HAc-Alg/30 wt% CaP hydrogels. (B) Cell viability of HAc-Alg and HAc-Alg/30 wt% CaP hydrogels measured by AlamarBlue assay after 3 and 5 days (n > 3, **P < 0.01). (C) Confocal laser scanning microscope (CLSM) z-stack images of L929 fibroblasts adhered to HAc-Alg/30wt% CaP three-dimensional (3D) scaffolds after 7 days, indicating the selected parts for imaging. The two layers from the bottom and center regions were imaged to confirm the cell distribution throughout the scaffold. (D) CLSM z-stacked confocal images of L929 fibroblasts within the 3D printed scaffold after a 14-day incubation period.