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SEM images and EDS spectra of (A) HAc-Alg and (B) HAc-Alg/30wt% CaP. HAc-Alg contains a significant amount of calcium due to crosslinking of alginate as well as the existence of calcium carbonate. Because of calcium in alginate, the ratio of Ca/P in HAc-Alg/30wt% CaP is found to be ~1.6. Thus, the Ca/P ratios of CaP nanoprecipitates should be determined using TEM and corresponding EDS analysis instead of SEM.
