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Sequence of primers for the RT-qPCR.
| Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| Actin | 5’-GTGCTATGTTGCCCTAGACTTCG-3’ | 5’-GATGCCACAGGATTCCATACCC-3’ |
| Col 1 | 5’-CAAGATGTGCCACTCTGACT-3’ | 5’-TCTGACCTGTCTCCATGTTG-3’ |
| RunX2 | 5’-GCATGGCCAAGAAGACATCC-3’ | 5’-CCTCGGGTTTCCACGTCTC-3’ |
| OCN | 5’-CTTTCTGCTCACTCTGCTG-3’ | 5’-TATTGCCCTCCTGCTTGG-3’ |
| OPN | 5’-CACTTTCACTCCAATCGTCCCTAC-3’ | 5’-ACTCCTTAGACTCACCGCTCTTC-3’ |
RT-qPCR: Quantitative real-time polymerase chain reaction, OCN: Osteocalcin, OPN: Osteopontin
